A FlAsH–Tetracysteine Assay for Quantifying the Association and Orientation of Transmembrane α-Helices

Authors

• Christopher J. Pace
• Qiongying Huang
• Fang Wang
• Kanwal S. Palla

Document Type

Article

Publication Date

5-2-2011

Publisher

John Wiley & Sons, Inc

Abstract

Lighting up protein dimers in membranes: The association of membrane proteins plays a critical role in biology. We describe a FlAsH–tetracysteine (tC) assay that detects protein dimerization in lipid bilayers with a fluorescent readout. The stringent spatial requirement of FlAsH–tC complex formation readily differentiates helix dimers in parallel and antiparallel orientations.

Recommended Citation

Pace, C. J., Huang, Q., Wang, F., Palla, K. S., Fuller, A. A., & Gao, J. (2011). A FlAsH–Tetracysteine Assay for Quantifying the Association and Orientation of Transmembrane α-Helices. ChemBioChem, 12(7), 1018–1022. https://doi.org/10.1002/cbic.201000736