Document Type
Article
Publication Date
10-18-2016
Publisher
American Chemical Society
Abstract
The first report of a water-soluble peptoid adsorbed to silica monitored by second harmonic generation (SHG) at the liquid/solid interface is presented here. The molecular insights gained from these studies will inform the design and preparation of novel peptoid coatings. Simple 6- and 15-residue peptoids were dissolved in phosphate buffered saline and adsorbed to bare silica surfaces. Equilibrium binding constants and relative surface concentrations of adsorbed peptoids were determined from fits to the Langmuir model. Complementary fluorescence spectroscopy studies were used to quantify the maximum surface excess. Binding constants, determined here by SHG, were comparable to those previously reported for cationic proteins and small molecules. Enthalpies and free energies of adsorption were determined to elucidate thermodynamic driving forces. Circular dichroism spectra confirm that minimal conformational changes occur when peptoids are adsorbed to silica while pH studies indicate that electrostatic interactions impact adsorption.
Recommended Citation
Calkins, A. L., Yin, J., Rangel, J. L., Landry, M. R., Fuller, A. A., & Stokes, G. Y. (2016). A Thermodynamic Description of the Adsorption of Simple Water-Soluble Peptoids to Silica. Langmuir, 32(44), 11690–11697. https://doi.org/10.1021/acs.langmuir.6b02804
Comments
This is an open access article which permits copying and redistribution of the article or any adaptations for non-commercial purposes.