Date of Award

6-2023

Document Type

Thesis - SCU Access Only

Publisher

Santa Clara : Santa Clara University, 2023

Department

Bioengineering

First Advisor

Bill Lu

Abstract

Exosomes are small extracellular lipid vesicles. Exosomes have a number of exploitable properties that counteract the traditional limitations of protein therapeutics, namely penetration through the blood brain barrier and into solid-body tumors. This would allow for the scavenger treatment of various neurological conditions caused by aberrant proteins, such as Alzheimer’s Disease. In this work, we describe the modification of exosomes to surface express scavenging binding proteins. The engineered exosome system uses fluorimetric and chemiluminescent markers in embedded recombinant proteins to verify production and function. The system uses two reporters to verify functionality of the exosomes, one with a binding epitope specific to the binding protein on the exosome. Further testing is needed to confirm the specificity through a binding assay.

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