Date of Award

Spring 2022

Document Type

Thesis

Publisher

Santa Clara : Santa Clara University, 2022.

Degree Name

Master of Science (MS)

Department

Bioengineering

First Advisor

Zhiwen Zhang

Abstract

Facing the danger of Gram-positive bacterial infection, mammalian cells have their defense line. A functional proteomics study aided with Deep Learning indicates the direct interaction between collagen V from mammalian cell membrane and Sortase A which catalyzes the display of virulence factors on the gram positive bacteria. And CHO cell proteomes conducted with the use artificial intelligence via Tensorflow and found that the identified anti collagen V binds to Sortase A of S. aureus and regulates its monomer-homodimer equilibrium, which is responsible for the bacterial cell wall sorting activity. Our results suggest that the collagen V plays an instrumental role in inhibiting Gram-positive bacterial infections and impeding their pathogenicity.

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