Date of Award
Santa Clara : Santa Clara University, 2019.
Sortase A is a transmembrane protein prominent in gram-negative bacterial strains. It is a virulence factor that anchors other proteins, which facilitate MRSA infections. In the long term, we plan to utilize this protein to create anti-infective drugs as antibiotic resistance continues to become a global health issue. Starting with five NIH drug candidates, we decided to first study Sortase A with B12 due to its historical past of ancient civilizations using natural sources of B12 to treat infections. Our senior design is further centered around understanding how vitamin B12 interacts with Sortase A in vitro, particularly the binding affinity between the two. The in vitro experiments will verify the previous in vivo studies.
Chau, Huong; Matsuda, Alice; and Johar, Leepakshi, "Anti-Infective Mechanism-Based Drug Discovery via Sortase A" (2019). Bioengineering Senior Theses. 81.