Date of Award

6-7-2019

Document Type

Thesis

Publisher

Santa Clara : Santa Clara University, 2019.

Department

Bioengineering

First Advisor

Zhiwen Zhang

Abstract

Sortase A is a transmembrane protein prominent in gram-negative bacterial strains. It is a virulence factor that anchors other proteins, which facilitate MRSA infections. In the long term, we plan to utilize this protein to create anti-infective drugs as antibiotic resistance continues to become a global health issue. Starting with five NIH drug candidates, we decided to first study Sortase A with B12 due to its historical past of ancient civilizations using natural sources of B12 to treat infections. Our senior design is further centered around understanding how vitamin B12 interacts with Sortase A in vitro, particularly the binding affinity between the two. The in vitro experiments will verify the previous in vivo studies.

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