Date of Award
Santa Clara University
Recent research advancements in induced pluripotent stem cells (iPSCs) have broadened their capacity for research in specific disease models3 need for effective and robust techniques to detect cell differentiation. Two techniques currently exist to determine cell type; morphology and immunostaining. Although they have been used by researchers for many years, these methods can be unreliable, time-consuming, and very uneconomical. We seek to create a technology that will be more accurate and efficient in detecting differentiation of iPSCs. This will allow researchers greater speed in screening for specific differentiation products, further increasing the utility of iPSCs in medical research. We will create an iPSC reporter cell line with the use of the Cas9/CRISPR associated novel genome editing technology. We aim to generate a reporter cell line that expresses green fluorescent protein only after neural differentiation.
Ito, Cade Ellis and Wolfe, Nick, "Genome Editing of Human iPSCs using the Cas9 System" (2014). Bioengineering Senior Theses. 12.