Increasing Protein Stability through Control of the Nanoscale Environment
American Chemical Society
We have discovered a novel property of single-walled carbon nanotubes (SWNTs)their ability to stabilize proteins at elevated temperatures and in organic solvents to a greater extent than conventional flat supports. Experimental results and theoretical analysis reveal that the stabilization results from the curvature of SWNTs, which suppresses unfavorable protein−protein lateral interactions. Our results also indicate that the phenomenon is not unique to SWNTs but could be extended to other nanomaterials. The protein−nanotube conjugates represent a new generation of active and stable catalytic materials with potential use in biosensors, diagnostics, and bioactive films and other hybrid materials that integrate biotic and abiotic components.
Asuri, P.; Karajanagi, S.S.; Yang, H.; Yim, T.-J.; Kane, R.S.; Dordick, J.S. Increasing protein stability through control of the nanoscale environment, Langmuir, 22, 5833-5836 (2006).